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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
| Immunology and Serology | 5-10% | - Infectious disease serology
- Autoimmune and immunodeficiency disorders
- Immunologic testing methodologies
- Immune system principles and components
|
| Microbiology | 15-20% | - Mycology, parasitology, and virology
- Identification of pathogenic microorganisms
- Bacteriology and antimicrobial susceptibility
- Specimen collection and culture techniques
|
| Laboratory Operations | 5-10% | - Quality control and quality assurance
- Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
- Laboratory safety and regulatory compliance
|
| Clinical Chemistry | 20-25% | - Toxicology and liver/kidney function tests
- Endocrinology and therapeutic drug monitoring
- Carbohydrates, lipids, proteins, and enzymes
- Electrolytes, acid-base balance, and blood gases
|
| Urinalysis and Other Body Fluids | 5-10% | - Urine formation and composition
- Semen and amniotic fluid testing
- Physical, chemical, and microscopic analysis
- Cerebrospinal, serous, and synovial fluids
|
| Hematology | 20-25% | - Routine and automated hematology procedures
- Hemostasis and coagulation testing
- White blood cell morphology and diseases
- Red blood cell physiology and disorders
|
| Blood Banking (Immunohematology) | 15-20% | - Compatibility testing
- Blood group systems and antigens
- Blood components and storage
- Antibody detection and identification
- Transfusion reactions and adverse events
|
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. HbsAg is positive in acute and chronic Hepatitis B infections, since the antigen is found on the actual surface of the virus. HbeAg is present in the blood when the hepatitis B viruses are replicating, indicating an active infection. Anti-Hbc IgM is present due to the immune response to the presence of the hepatitis core antigen and indicates an acute infection. Anti-HBs is generally interpreted as indicating recovery and immunity from hepatitis B virus infection, according to the CDC.
Given the following results, what is the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative
A) susceptible
B) immunization
C) acute infection
D) chronic infection
2. Bone marrow biopsies are helpful diagnostic tools in:
Evaluation of anemia and thrombocytopenia
Evaluation of cytopenias
Diagnosing leukemias
Can be part of evaluation for FUO
Bone marrow biopsies are NOT used as screens for hematologic issues during routine check-ups.
Hematology
In which of the following situations would a bone marrow aspirate and biopsy possibly be used as an aid for diagnosis? (Select all that apply)
A) Evaluation of anemia and thrombocytopenia
B) Evaluation of fever of unknown origin (FUO)
C) Evaluation of cytopenias
D) Diagnosis of leukemias
E) Screening for hematologic issues during routine check-ups
3. The federal government has categorized critical biological agents into three groups: A, B, and C. Those agents in category A are highest-priority because they can be easily disseminated or transmitted person-to-person.
The federal government has categorized critical biological agents into three groups: A, B, and C. Those agents in category A are highest-priority because they:
A) Cause moderate morbidity and low mortality.
B) Could be engineered for mass dissemination at some future date.
C) Can be easily disseminated or transmitted person-to-person.
4. Which of the following is NOT part of the magnification system of the microscope?
A) 10 X objective lens
B) 4 X objective lens
C) ocular lenses-arm
D) arm
5. Fusarium species is the most likely associated with mycotic keratitis.
Trichophyton rubrum is a dermatophyte that commonly causes an itching, scaling skin infection of the feet, known as tinea pedis. Scedosporium apiospermum is commonly associated with sinusitis. Aspergillus niger typically causes otitis externa and can also be associated with sinusitis.
Which of the following species or organisms is the most likely to be the cause of mycotic keratitis (fungal eye infection)?
A) Aspergillus niger
B) Trichophyton rubrum.
C) Fusarium species
D) Scedosporium apiospermum
Solutions:
Question # 1 Answer: C | Question # 2 Answer: A,B | Question # 3 Answer: C | Question # 4 Answer: D | Question # 5 Answer: C |